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W

吴旻

邮          箱：wumin@whu.edu.cn

职          称：教授

办公室地址：

实验室地址：武汉大学生命科学学院5025

个人简介

个人介绍

长期致力于利用细胞分子生物学和表观遗传组学相关技术，研究肿瘤和免疫相关疾病的表观遗传学调控机制，发现异染色质异常可以诱导核DNA和组蛋白转运到细胞质的细胞生物学现象，揭示了组蛋白甲基化H3K36甲基化修饰及其修饰酶SETD2在肿瘤发生发展过程中的作用及其分子调控机制，以及增强子在乳腺癌和结直肠癌等癌症中的动态变化规律及其作用机制。现为教育部免疫与代谢前沿科学中心成员，湖北省细胞稳态重点实验室和胎源性疾病重点实验室成员，曾获国家自然科学奖二等奖一项（第四完成人），于2011年获教育部“新世纪优秀人才计划”支持。

教育经历

1995.9-1999.6 北京大学生命科学学院，本科生，获学士学位

1999.9-2004.6 北京大学生命科学学院，硕博连读研究生，获博士学位

2001.3-2002.6 美国国立犹太医学研究中心，交换学生

工作经历

2004.9-2007.3 耶鲁大学医学院，博士后

2007.4-2009.2 美国Stowers医学研究所，博士后

2009.3-至 今 武汉大学生命科学学院，教授，博士生导师

荣誉奖励

2022 乳腺癌基因诊断指导精准治疗关键技术创新与应用，湖北省科技进步二等奖，第四完成人

2016 武汉大学生命科学学院“双一流”特聘教授

2010 细胞凋亡与抗病毒反应的信号转导研究，国家自然科学二等奖，第四完成人

2010 湖北省“楚天学者”计划楚天学子教授

2006 北京大学优秀博士论文二等奖

2006 细胞凋亡与NF-kappaB激活的信号传导研究，教育部科学技术奖一等奖，第四完成人

社会服务

2015.4至今 中国生物化学与分子生物学会基因专业委员会委员

2016.6至今 中国细胞生物学会染色质生物学分会委员

2017.7至今 湖北省细胞生物学会理事

2021.10至今 中国医药生物技术协会疾病模型专业委员会委员

科研领域

癌症发生发展的过程是遗传信息和癌细胞周围环境相互作用的结果，遗传和表观遗传信息的共同作用影响了细胞的各种表型。从正常细胞转变为癌细胞的过程中，基因组上的表观遗传信息发生了巨大的变化，多个相关基因在癌组织中频繁发生突变，DNA甲基化和组蛋白修饰等表观遗传修饰也发生规律性变化。通过发现癌细胞中特异的表观遗传现象，阐明其内在的分子机制，可以发展用于癌症诊断和治疗的新型靶点和策略。目前，多种基于表观遗传机制的药物和方法已经用于癌症的临床诊断和治疗中。本实验室致力于研究癌症发生发展过程中的表观遗传调控机制，运用分子生物学、细胞生物学、表观遗传组学和生物信息学的方法探究表观遗传因子在肿瘤发生发展和肿瘤微环境中的作用和分子机制，阐明癌细胞中转录因子和表观遗传之间的调控网络，特别关注基因组上组蛋白修饰的动态变化在癌细胞转化、增殖、转移和炎症反应过程中的作用，探索研发可用于癌症诊断和治疗的新方法和新药物。

代表性论文

  1. Xiao, Q.#, Xiao, Y., Li, L.Y., Chen, M.K.*, andWu, M.*, Multifaceted regulation of enhancers in cancer. Biochim Biophys Acta Gene Regul Mech, 2022. 1865(6): p. 194839.

  2. Chen, L.#, Yang, C., Tang, S.-B., Long, Q.-Y., Chen, J.-D.,Wu, M.*, and Li, L.-Y.*, Inhibition of histone methyltransferase SETD8 represses DNA virus replication. Cell Insight, 2022. 1(3).

  3. Chen, L.#, Luo, Z.#, Zhao, C.#, Li, Q., Geng, Y., Xiao, Y., Chen, M.K., Li, L., Chen, Z.X.*, andWu, M.*, Dynamic Chromatin States Coupling with Key Transcription Factors in Colitis-Associated Colorectal Cancer. Adv Sci (Weinh), 2022: p. e2200536.

  4. Li, Q.L.#, Lin, X.#, Yu, Y.L.#, Chen, L.#, Hu, Q.X., Chen, M., Cao, N., Zhao, C., Wang, C.Y., Huang, C.W., Li, L.Y., Ye, M. *, andWu, M.*, Genome-wide profiling in colorectal cancer identifies PHF19 and TBC1D16 as oncogenic super enhancers. Nat Commun, 2021. 12(1): p. 6407.

  5. Hu, Q.X.#, Wang, H.Y., Jiang, L., Wang, C.Y., Ju, L.G., Zhu, Y., Zhong, B.,Wu, M.*, Wang, Z. *, and Li, L.Y. *, Histone demethylase LSD1 promotes RIG-I poly-ubiquitination and anti-viral gene expression. PLoS Pathog, 2021. 17(9): p. e1009918.

  6. (6)Wang, Z.#, Chen, J., Gao, C., Xiao, Q., Wang, X.W., Tang, S.B., Li, Q.L., Zhong, B., Song, Z.Y., Shu, H.B., Li, L.Y., andWu, M.*, Epigenetic Dysregulation Induces Translocation of Histone H3 into Cytoplasm. Adv Sci (Weinh), 2021: p. e2100779.

  7. Wang, C.Y.#, Yu, G.T.#, Gao, C.#, Chen, J., Li, Q.L., Zhang, L.,Wu, M.*, Sun, Z.J. *, and Li, L.Y. *, Genome-Wide Enhancer Analysis Reveals the Role of AP-1 Transcription Factor in Head and Neck Squamous Cell Carcinoma. Front Mol Biosci, 2021. 8: p. 701531.

  8. Zheng, J.Y.#, Wang, C.Y., Gao, C., Xiao, Q., Huang, C.W.,Wu, M.*, and Li, L.Y. *, MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity. Cell Death Dis, 2021. 12(4): p. 364.

  9. Xu, L.#, Zheng, Y., Li, X., Wang, A., Huo, D., Li, Q., Wang, S., Luo, Z., Liu, Y., Xu, F., Wu, X.,Wu, M.*, and Zhou, Y. *, Abnormal neocortex arealization and Sotos-like syndrome-associated behavior in Setd2 mutant mice. Sci Adv, 2021. 7(1).

  10. Li, X.J.#, Li, Q.L., Ju, L.G., Zhao, C., Zhao, L.S., Du, J.W., Wang, Y., Zheng, L., Song, B.L., Li, L.Y., Li, L. *, andWu, M.*, Deficiency of Histone Methyltransferase SET Domain-Containing 2 in Liver Leads to Abnormal Lipid Metabolism and HCC. Hepatology, 2021. 73(5): p. 1797-1815.

  11. Yao J.#, Lei P.J.#, Li Q.L.#, Chen J., Tang S.B., Xiao Q., Lin X., Wang X., Li L.Y.,Wu M.* GLIS2 promotes colorectal cancer through repressing enhancer activation. Oncogenesis 2020, 9:57.

  12. (12)Gao C.#, Chen L.#, Tang S.B.#, Long Q.Y.#, He J.L., Zhang N.A., Shu H.B., Chen Z.X.,Wu M.*, Li L.Y.* The epigenetic landscapes of histone modifications on HSV-1 genome in human THP-1 cells. Antiviral Res 2020, 176:104730.

  13. Wang H.Y.#, Long Q.Y.#, Tang S.B., Xiao Q., Gao C., Zhao Q.Y., Li Q.L., Ye M., Zhang L., Li L.Y.,Wu M.* Histone demethylase KDM3A is required for enhancer activation of hippo target genes in colorectal cancer.Nucleic Acids Res2019, 47:2349-2364.

  14. Li Q.L.#, Wang D.Y.#, Ju L.G., Yao J., Gao C., Lei P.J., Li L.Y., Zhao X.L.*,Wu M.* The hyper-activation of transcriptional enhancers in breast cancer.Clin Epigenetics2019, 11:48.

  15. Ju L.G.#, Zhu Y., Long Q.Y., Li X.J., Lin X., Tang S.B., Yin L., Xiao Y., Wang X.H., Li L., Zhang L.,Wu M.* SPOP suppresses prostate cancer through regulation of CYCLIN E1 stability.Cell Death Differ2019, 26:1156-1168.

  16. 王震#，李联运*，吴旻*。表观遗传相关药物对癌症的治疗。细胞生物学报，2018;40(8):1253-1263.

  17. Zhu K.#, Lei P.J.#, Ju L.G.#, Wang X., Huang K., Yang B., Shao C., Zhu Y., Wei G., Fu X.D., Li L.,Wu M.* SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing.Nucleic Acids Res2017, 45:92-105.

  18. Wang Z.#, Long Q.Y., Chen L., Fan J.D., Wang Z.N., Li L.Y.*,Wu M.*, Chen X.* Inhibition of H3K4 demethylation induces autophagy in cancer cell lines.Biochim Biophys Acta2017, 1864:2428-2437.

  19. Li Q.L.#, Lei P.J.#, Zhao Q.Y.#, Li L., Wei G.*,Wu M.* Epigenomic analysis in a cell-based model reveals the roles of H3K9me3 in breast cancer transformation.Epigenomics2017, 9:1077-1092.

  20. Ju L.G.#, Zhu Y.#, Lei P.J., Yan D., Zhu K., Wang X., Li Q.L., Li X.J., Chen J.W., Li L.Y.*,Wu M.* TTLL12 Inhibits the Activation of Cellular Antiviral Signaling through Interaction with VISA/MAVS.J Immunol2017, 198:1274-1284.

  21. Jia X.,#Long Q., Miron R.J., Yin C., Wei Y., Zhang Y.*,Wu M.* Setd2 is associated with strontium-induced bone regeneration.Acta Biomater2017, 53:495-505.

  22. Zhao Q.Y.#, Lei P.J.#, Zhang X., Zheng J.Y., Wang H.Y., Zhao J., Li Y.M., Ye M., Li L., Wei G.*,Wu M.* Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model.Clin Epigenetics2016, 8:34.

  23. Su S.K.#, Li C.Y., Lei P.J., Wang X., Zhao Q.Y., Cai Y., Wang Z., Li L.*,Wu M.* The EZH1-SUZ12 complex positively regulates the transcription of NF-kappaB target genes through interaction with UXT.J Cell Sci2016, 129:2343-2353.

  24. Zhu K.#, Wang X., Ju L.G., Zhu Y., Yao J., Wang Y.,Wu M.*, Li L.Y.* WDR82 Negatively Regulates Cellular Antiviral Response by Mediating TRAF3 Polyubiquitination in Multiple Cell Lines.J Immunol2015, 195:5358-5366.

  25. Fan J.D.#, Lei P.J., Zheng J.Y., Wang X., Li S., Liu H., He Y.L., Wang Z.N., Wei G., Zhang X., Li L.Y.*,Wu M.* The selective activation of p53 target genes regulated by SMYD2 in BIX-01294 induced autophagy-related cell death.PLoS One2015, 10:e0116782.

  26. Wang X.#, Ju L., Fan J., Zhu Y., Liu X., Zhu K.,Wu M.*, Li L.* Histone H3K4 methyltransferase Mll1 regulates protein glycosylation and tunicamycin-induced apoptosis through transcriptional regulation.Biochim Biophys Acta2014, 1843:2592-2602.

  27. Zhao Q.#, Fan J., Hong W., Li L.,Wu M.* Inhibition of cancer cell proliferation by 5-fluoro-2'-deoxycytidine, a DNA methylation inhibitor, through activation of DNA damage response pathway.Springerplus2012, 1:65.

  28. Wang X.#, Zhu K.#, Li S., Liao Y., Du R., Zhang X., Shu H.B., Guo A.Y., Li L.*,Wu M.* MLL1, a H3K4 methyltransferase, regulates the TNFalpha-stimulated activation of genes downstream of NF-kappaB.J Cell Sci2012, 125:4058-4066.

  29. Wu M.*, Shu H.B.* MLL1/WDR5 complex in leukemogenesis and epigenetic regulation.Chin J Cancer2011, 30:240-246.

  30. Wu M.#, Wang P.F.#, Lee J.S., Martin-Brown S., Florens L., Washburn M., Shilatifard A. Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.Mol Cell Biol2008, 28:7337-7344.

  31. Higa L.A.#,Wu M.#, Ye T., Kobayashi R., Sun H., Zhang H. CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation.Nat Cell Biol2006, 8:1277-1283.

  32. Banks D.#,Wu M.#, Higa L.A., Gavrilova N., Quan J., Ye T., Kobayashi R., Sun H., Zhang H. L2DTL/CDT2 and PCNA interact with p53 and regulate p53 polyubiquitination and protein stability through MDM2 and CUL4A/DDB1 complexes.Cell Cycle2006, 5:1719-1729.

  33. Wu M.#, Xu L.G., Su T., Tian Y., Zhai Z., Shu H.B. AMID is a p53-inducible gene downregulated in tumors.Oncogene2004, 23:6815-6819.

  34. Wu M.#, Xu L.G., Zhai Z., Shu H.B. SINK is a p65-interacting negative regulator of NF-kappaB-dependent transcription.J Biol Chem2003, 278:27072-27079.

  35. Xu L.G.#,Wu M.#, Hu J., Zhai Z., Shu H.B. Identification of downstream genes up-regulated by the tumor necrosis factor family member TALL-1.J Leukoc Biol2002, 72:410-416.

  36. Wu M.#, Xu L.G.#, Li X., Zhai Z., Shu H.B. AMID, an apoptosis-inducing factor-homologous mitochondrion-associated protein, induces caspase-independent apoptosis.J Biol Chem2002, 277:25617-25623.

实验室简介

研究方向 肿瘤表观遗传学 邮箱 wumin@whu.edu.cn
职称 教授 办公室地址
实验室地址 武汉大学生命科学学院5025

地址：湖北武汉武昌区东湖路115号    邮编：430071

邮箱：office.tkms@whu.edu.cn

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